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ASCP ASCP-MLT Exam Syllabus Topics:

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. n reporting out Gram stains, one should not go beyond what objective observation will allow. In this case, bacterial cells are observed, which are spherical and gram-positive. Their arrangement in tetrads and clusters might be helpful to the physician, suggesting staphylococci. However, one should stop short of naming staphylococci in an official report, as the large gram-positive cocci in tetrads is also suggestive of Micrococcus species, for which human infections have not been reported.
A Gram stain of the serous exudate is shown in the image. The appropriate report would read:

A) Gram-positive cocci in tetrads and clusters
B) Smear positive for bacteria
C) Gram-positive cocci
D) Gram-positive cocci suggestive of Staphylococcus species

2. Listeria monocytogenes is the correct answer. The motility agar is showing motility at the top of the tube, but not deeper; typical of this catalase-positive, gram positive bacillus. Streptococcus agalactiae would be catalase negative and a coccus. Erysipelothrix rhusiopathiae would be H2S-positive and catalase negative. Escherichia coli is a gram negative bacillus.

This Gram-positive bacillus grew as a diffusely beta-hemolytic colony from a newborn. It was catalase positive and had tumbling motility on a hanging drop preparation. This is how it appeared on triple sugar iron agar and motility medium. What is the most likely diagnosis?

A) Listeria monocytogenes
B) Escherichia coli
C) Erysipelothrix rhusiopathiae
D) Streptococcus agalactiae

3. The organisms in this image are demonstrating a gram-variable phenomenon. For the most part, the organisms are staining primarily gram-negative, however, on the tips of some of the rods, there is a gram-positive staining morphology. This can be defined as gram-variable.
Which of the following best describes the organisms seen in this illustration:

A) gram-positive
B) gram-variable
C) acid-fast
D) gram-negative

4. IgE levels are often increased in patients with allergic disease. IgE binds to the membranes of mast cells and basophils, and if specific antigen is present to react with the IgE molecule, degranulation of these cells occurs, releasing histamines, and other substances into the blood or tissues.
Which of the following immunoglobulin classes is chiefly responsible for the degranulation of mast cells and basophils:

A) IgM
B) IgG
C) IgA
D) IgE

5. The second choice is correct. Standard Precautions means to treat blood, all body fluids, all secretions (except sweat), all excretions, non-intact skin, and mucous membranes as if they were infectious. The purpose of this rule is to ensure the safety of and to protect those who come into contact with potentially infectious material.
Which one statement about Standard Precautions is most correct?

A) Standard Precautions do not apply to pleural fluid or semen.
B) Standard Precautions means to treat blood, all body fluids, all secretions (except sweat), all excretions, non-intact skin, and mucous membranes as if they were infectious.
C) Standard Precautions do not apply to feces or urine.
D) Standard Precautions means to treat all patients as if they were infectious.

Solutions: