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American Society of Microbiology ABMM Sample Questions:

1. Under the Clinical Laboratory Improvement Amendments (CLIA), participation in an approved external Proficiency Testing (PT) program serves primarily to:

A) Determine the reference intervals (normal ranges) for quantitative assays performed by the laboratory.
B) Provide an objective, ongoing assessment of the accuracy and reliability of the laboratory's testing compared to its peers.
C) Fulfill requirements for laboratory accreditation by The Joint Commission only.
D) Validate the performance characteristics (e.g., sensitivity, specificity) of newly implemented test methods.

2. A microbiology laboratory receives a corneal scraping from a patient with keratitis. Microscopic examination with calcofluor white stain reveals the presence of septate hyphae and conidiophores with a foot cell and phialides. The MOST likely genus is:

A) Candida
B) Aspergillus
C) Rhizopus
D) Mucor

3. A patient with a history of neutropenia following chemotherapy develops a rapidly progressive pneumonia.
Bronchoalveolar lavage fluid reveals septate hyphae with acute angle (45-degree) branching. The MOST likely diagnosis is invasive pulmonary aspergillosis caused by:

A) Fusarium species
B) Rhizopus species
C) Aspergillus fumigatus
D) Mucor species

4. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

A) The number of true negative results divided by the total number of true negative and false positive results.
B) The number of true positive results divided by the total number of positive test results.
C) The number of true positive results divided by the total number of true positive and false negative results.
D) The number of true negative results divided by the total number of negative test results.

5. A fungal isolate grown from a blood culture at 37°C shows budding yeast cells. When subcultured to cornmeal agar with Tween 80 and incubated at 25°C, it produces pseudohyphae and terminal chlamydospores.
Growth on CHROMagar Candida yields green colonies. This organism is most likely:

A) Candida parapsilosis
B) Candida glabrata
C) Cryptococcus neoformans
D) Candida albicans

Solutions: